Flexitube sirna manual

Article Snippet: siRNA Transfection MCF7 cells in well tissue culture plates for RT-qPCR or six-well tissue culture plates for western blot analysis were transiently transfected with HiPerfect transfection reagent (Qiagen) using 75 ng of a CXXC5 siRNA (FlexiTube GeneSolution GS, Qiagen). Nucleofector Kit V (Lonza). siRNA ( nM) targeting NOL7 or control (non-targeting) siRNA (Flexitube siRNA, Qiagen GmbH, Hilden, Ger-many) were used. Non-transfected cells and con-trol siRNA-transfected cells were used as negative controls. Each siRNA treatment was performed in triplicate. After transfection, 1 μM ATRA (Sigma. Two different FlexiTube products are available: FlexiTube siRNA and FlexiTube GeneSolution. FlexiTube siRNA is a cost-effective solution for RNAi analysis of small numbers of genes. siRNAs are provided in 5 nmol or 20 nmol amounts for human, mouse, or rat genes, or in economical 1 nmol amounts for human and mouse genes.

The siRNA oligonucleotides targeting Arf1 and IRSp53 were purchased from Qiagen (siARF1#1, Hs_BCAR1_5 FlexiTube siRNA, cat. no. SI; and siBCAR1#6, Hs_BCAR1_6 FlexiTube siRNA, cat. no. After overnight incubation, cells were treated using Allstars Negative Control siRNA (Neg. Co., #SI) and specific FlexiTube siRNAs targeting FZD6 (FZD6_7, #SI; FZD6_8, #SI). Transfection was conducted using INTERFERin™ (Polyplus, Illkirch, France) according to the manufacturer´s recommendation. The siRNAs targeted against the human ALK4 (siRNA Hs_ALK4_5 FlexiTube siRNA, SI, siRNA Hs_ALK4_6 FlexiTube siRNA, SI), FOXO3a (Hs_FOXO3a_3 FlexiTube siRNA.

11 авг. г. Upon siRNA-mediated knockdown of IKKε, downregulation of PSAT1 at the FlexiTube siRNA (S, Qiagen); Hs_IKBKE_8 FlexiTube siRNA. Table Custom FlexiTube siRNA assay IDs. Table siRNA optimization kits according to the manufacturers' instructions. FlexiTube siRNA range (SI, Qiagen; siRNA sequence: 30 min over 24 h. Cells were manually tracked using the MTrackJ plugin for ImageJ (http://.